Definition
Branch of biotechnology in which plant cell ,tissue ,organ, or small ,pieces of plants are grown artificially under sterile and controlled laboratory condition on the nutrient culture media.
Aim
To produce a large number of genetically identical, disease-free plants in a short period of time, independent of seasonal and environmental conditions.
Historical background
Gottlieb Haberlandt known as father of plant tissue culture. He termed the coin Totipotency in plants which means the plant has the ability to grow a new plant from a single cell, as the plant cell transfer complete genetic information where as in animals totipotency is absent.
Introduction
As an intern ,to study the proper handling of the equipment ,instrumentation and learn the skill required for PTC.
To study in detail the step by step procedure for PTC.
To understand the requirement ,and reasoning for the preparation of the media stock.
To observe the growth of the cultured explant.
Objectives
To understand the technique performed to propagate better quality of plant material for betterment of mankind as well as rapid multiplication of the required or endangered plant species irrespective of any weather condition under sterile condition.
-We can grow a completely disease free planting material.
-Year round production.
-Genetic improvement is done on various species.
9/5/26
Laborator safety, Handling of the lab instruments used for sterilization and what precautions should be taken before you start
-loading
1] Lab hot air oven
It sterilizes glassware and dries samples using dry heat.
Procedure:
-loading
-setting parameters accordingly
-operation
-unloading and cooling
-safety and maintenance
2]Autoclave
it is a moist heat sterilizer .It uses saturated steam under pressure to kill bacteria, fungi, spores. That’s why we use it for media, water ,and glassware before plant tissue culture.
procedure:
-prep items
-loading
-setting parameters
-operation
-Unloading and cooling
-safety checks
10/5/26
1]Handling of Laminar air flow and precautions before and during its use.
-used for aseptic work in plant tissue culture, media pouring, inoculation. It gives a stream of HEPA-filtered air to keep your working zone sterile.
2]Use of incubator
-It gives controlled temperature, humidity, and sometimes, carbon dioxide, for growing microbes, plant tissue cultures, seeds, or keeping samples at a set temperature .No sterilization, only growth.
3]Discarding procedure of the used petriplates.
4]Cleaning of glassware
5]other activity(isolation of bacteria and fungi decomposed sample)
11/5/26
1]Theory lecture on Plant tissue culture by Sayali Ma’am.
-Application, advantages, and limitations of PTC.
-Laboratory safety in PTC
-other activity(making of H2S kit for water testing.
12/5/26
Preperation of stock for MS media preparation.
stock components Function
- Macronutrients required in large quantity
2. Micronutrients required in small quantity
3. Iron source chlorophyll formation
4. Vitamins
5.Carbon source (sucrose)
6.Plant hormones
7.Agar
8.Distilled water
