Streaking plate method

Streaking plate

Material – 1.Bacterial culture or previously poured bacterial agar plate. 2.Inoculation loop. 3. Lighter. 4. Bunsen burner. 5. Ethanol. 6. Agar plate. 6. Tissue paper.

Procedure –

Firstly sterilized the surface area of laminar and then put the bacterial plate , inoculation loop, lighter, Bunsen burner into the laminar.
Then sterilized the inoculating look Bunsen burner by putting the loop into the flame until red hot and allow it to cool
Then pick an isolated colony from the previously agar plate culture and spread it over the first quadrant ( approximately 1/4 the plate) using close parallel streak.
Immediately streak the inoculating loop very gently quarter of the plate using a back and fourth motion.
Then flame the loop again and allow it to cook and going back to the first area that just streaked extend the streaks into the second quarter of the plate.
Above process reapeted up to 3 and 4 quater.
Flame the loop once again
Then streaked plates are real by using parafilm and put in the incubator (37oc for 24 hrs.)