Activity No.1

Preparation of H2S Water kit

14/11/2025

Introduction

H₂S (Hydrogen Sulfide) test kits are widely used for the rapid detection of bacterial contamination in water. These kits work by indicating the presence of H₂S-producing bacteria, which are often associated with fecal contamination. The test is simple, reliable, and suitable for both laboratory and field applications.

  • Common bacteria associated with fecal contamination include E. coli, Salmonella, and H₂S-producing bacteria.
  • Drinking or using water with fecal contamination can cause waterborne diseases like diarrhea, cholera, typhoid.

Materials

  • Whatman Filter Paper No. 41
  • Prepared H₂S media
  • 50 plastic bottles (30 ml each, new)
  • Bottle caps
  • 70% Ethanol

Equipment

  • Laminar Air Flow (LAF) Cabinet
  • UV light (for sterilization in LAF)
  • Oven or incubator (50°C)
  • Forceps (sterile)
  • Sterile gloves
  • Scissors or paper cutter (for cutting strips)
  • Marker (for labeling bottles and strips)

H2S Media Preparation for 50 Bottle :

Name of ChemicalQuantity (gm/ml)
Bacteriological grade peptone20 gm
Di – potassium hydrogen phosphate1.5 gm
Ferric ammonium citrate0.75 gm
Sodium Thiosulphate1.0 gm
L-cysteine HCL0.125 gm
Liquid soap1.0 ml
Distilled water50 ml

Procedure

Step 1: Preparation of Test Strips

  1. Take Whatman Filter Paper No. 41.
  2. Cut the paper into strips of 8 cm × 2.5 cm using scissors or a paper cutter.
    • Ensure cuts are even to fit into the 30 ml bottles easily.
  3. Prepare the H₂S media in a clean, sterile container.
  4. Dip each paper strip completely into the media so it is fully saturated.
    • Handle strips only with sterile forceps to avoid contamination.
  5. Place all dipped strips on a clean tray to dry.
  6. Dry the strips in an oven at 50°C for 40 minutes.
    • This ensures that the media adheres to the paper and excess moisture is removed.
  7. After drying, transfer the strips into the laminar air flow (LAF) cabinet for sterilization.

Step 2: Sterilization of Plastic Bottles

  1. Take 50 new 30 ml plastic bottles.
  2. Clean the outer surface with 70% ethanol before placing them in the LAF.
  3. Place the bottles under UV light in the laminar air flow cabinet for 1 hour.
    • This step sterilizes the bottles and prevents bacterial contamination.
  4. After sterilization, keep bottles inside the LAF until ready for use.

Step 3: Loading Strips into Bottles

  1. Wear sterile gloves before handling strips or bottles.
  2. Use sterile forceps to pick up each dried H₂S strip.
  3. Place one strip per bottle.
    • Avoid touching the strip with your hands or the bottle walls.
  4. Secure the bottle with a tightly fitting cap immediately after placing the strip.
  5. Label each bottle with a unique ID or date for tracking.

Step 4: Post-Preparation Handling

  1. Store the prepared H₂S kits in a clean, dry place away from direct sunlight.
  2. Handle bottles carefully to prevent moisture or contamination from entering.
  3. Inspect bottles for any signs of contamination before use.

Precautions

  • Always work inside a laminar air flow cabinet when handling strips or bottles.
  • Use 70% ethanol to sanitize surfaces, equipment, and hands.
  • Wear sterile gloves at all times during the procedure.
  • Use sterile forceps for transferring strips.
  • Ensure bottle caps are tightly closed to prevent contamination.
  • Avoid prolonged exposure of strips to open air.
  • Regularly check UV lights for functionality to ensure sterilization is effective.

Workflow Summary (Step-by-Step Visual Guide)

  1. Cut Filter Paper → 8×2.5 cm strips
  2. Dip Strips → H₂S media
  3. Dry Strips → Oven 50°C, 40 min
  4. Sterilize Strips → Laminar Air Flow
  5. Sterilize Bottles → UV light, 1 hour in LAF
  6. Load Strips → Forceps, one strip per bottle
  7. Seal & Label → Tight caps, record ID/date

Activity No.2

Tital : p & G purifier of Water Packet Test

P&G Purifier of Water

Date: 28/11/2025

Name: P&G Purifier of Water

Net Content: 4 gm

One packet treats: 10 liters (2.5 gallons) of water

Active Ingredients

  1. Calcium Hypochlorite – 0.546%
  2. 2.17 ppm available chlorine

Purpose

Removes dirt, parasites, viruses, bacteria, and makes contaminated water safe to drink.

How to Use This Packet (Step-by-Step)

Step 1

  1. Take 10 liters of contaminated water in a clean bucket.
  2. Add one full 4 gm packet into the water.

Step 2

Stir the water vigorously for 5 minutes so the powder mixes properly.

Dirt and suspended particles start settling.

Leave water undisturbed for 5 minutes so heavy particles settle at the bottom.

Step 3 – Filter

  1. Without disturbing the settled dirt, pour the clear water through a clean cloth/cotton filter into another container.
  2. Wait 20 minutes after filtering.

Chlorine will disinfect the remaining microorganisms.

Step 4 – Drink

The water is now safe for drinking.
Do not drink if water is yellow in colour.

Advantages of P&G Purifier of Water

  1. Removes dirt and turbidity (makes muddy water clear).
  2. Heavy particles settle at the bottom.
  3. Kills germs and makes water safe.
  4. Contains chlorine that kills: Bacteria, Parasites, Harmful microorganisms
  5. Works on very polluted water: River water, Pond water, Flood water, Contaminated household water, Stored water
  6. Improves taste and smell after treatment.
  7. Water smells better and tastes fresher.
  8. Easy to use (simple 4-step process: Mix → Stir → Filter → Wait & drink).
  9. Quick process (Total purification time: ~25–30 minutes).
  10. Portable & lightweight.
  11. One small 4 gm packet can treat 10 liters of water.
  12. Cost-effective—replaces expensive purification systems for small quantities.
  13. Useful for traveling, camping, emergencies.
  14. Safe for emergency or disaster use (floods, electricity failure).
  15. Reduces waterborne diseases—helps prevent diarrhea, cholera, typhoid, dysentery.

Comparison

ParameterP & G Purifier of WaterRO Water Purifier
1) UseDesigned for emergency use, daily in low-income or disaster-hit areas, suitable for tap/river/borewell waterDesigned for daily use, commonly household use, suitable for tap/borewell water
2) Type of PurificationUses chemical treatment, chlorine dissolution, removes dirt, settles dirt at bottom, removes microorganismsUses reverse osmosis membrane, removes dissolved salts, chemicals, heavy metals, microorganisms
3) Speed2–3 min for 10 liters2–3 liters per minute
4) CostVery cheapHigh cost (machine + maintenance filters)
5) Power RequirementNo electricityElectricity required
6) Regular Use CaseEmergencies, outdoor fields, relief work, water contaminated with floodwaterRegular household drinking water use, areas with high TDS or chemical contamination

Observation Table :

SampleParameter (Before)P&G Purifier of Water (After)
Sample 1 (Grey water)TDS (ppm): 412
EC (m S/cm): 0824
pH: 8.06
Visual obs: Blackish water		TDS (ppm): 0461
EC (mS/cm): 0922
pH: 7.78
Visual obs: Clear water, no particles
Sample 2 (Tap water)TDS (ppm): 0255
EC (m S/cm): 0510
pH: 7.95
Visual obs: 		TDS (ppm): 0337
EC (mS/cm): 0675
pH: 7.15
Visual obs: Clear water
Sample 3 (Water mixed with soil)TDS (ppm): 0247
EC (m S/cm): 0494
pH: 8.19
Visual obs: Muddy or turbid water		TDS (ppm): 0340
EC (mS/cm): 0680
pH: 7.35
Visual obs: Clear water, no particles
Step 1
Step 2
Step 3
Step 4
Step 5

निष्कर्ष (Result):

या प्रयोगात P&G Purifier of Water वापरून वेगवेगळ्या प्रकारचे पाणी (Grey water, नळाचे पाणी व माती मिसळलेले पाणी) स्वच्छ करण्यात आले.

१. शुद्धीकरणानंतर सर्व नमुन्यांचे पाणी स्वच्छ, पारदर्शक व कणरहित झाले. यावरून हा purifier माती, घाण व गढूळपणा चांगल्या प्रकारे काढतो हे दिसून आले.

२.पाण्याचा pH 8 च्या आसपासून 7 ते 7.7 दरम्यान आला, म्हणजे पाणी पिण्यास अधिक सुरक्षित झाले.

३. शुद्धीकरणानंतर TDS व EC किंचित वाढले, कारण purifier मधील रसायनांमुळे काही घटक पाण्यात विरघळतात. ही वाढ हानिकारक नाही.

४. हा purifier पाण्यातील जंतू, घाण व सूक्ष्म कण नष्ट करून पाणी तात्काळ वापरण्यास योग्य करतो.

५. वीज न लागणारा, स्वस्त व जलद असल्यामुळे हा purifier आपत्कालीन परिस्थिती, पूरग्रस्त भाग, ग्रामीण भाग वापरासाठी खूप उपयुक्त आहे.

थोडक्यात निष्कर्ष:

P&G Purifier of Water पाणी स्वच्छ व सुरक्षित बनवतो आणि आपत्कालीन परिस्थितीत पिण्यायोग्य पाणी मिळवण्यासाठी अतिशय उपयुक्त आहे.

Activity No.3

Banana Plant Tissue Culture Training for DBRT Students

Date : 15 Dec 2025

Objective:

To provide hands-on training to DBRT students on the process of plant tissue culture using banana as a model plant.
The aim was to help students understand the principles, stages, and practical skills required to grow disease-free plants through tissue culture.

Date : 15 Dec 2025

I taught Plant Tissue Culture (Banana) to (Suhani , vansh , sahil ,gaytri ,Ruturaj,) students, explaining both theoretical and practical aspects.

Date : 16 Dec 2025

I demonstrated the use of Autoclave, Hot Air Oven, and pH Meter, and also explained how to discard contaminated culture bottles to Omkar, Suhani, Sahil, Gayatri, Payal and Ruturaj.
In addition, I taught them about Cactus Nursery Management — including which growing media should be used, how to measure the water-holding capacity of the soil, and how to cut and plant cactus cladodes properly.
All these topics were taught in detail, and the students also performed the practical sessions under my guidance.

Date: 28/12/2025

Today, I taught DBRT students (Suhani, Ruturaj, and Gayatri) the procedure for preparing MS medium required for Plant Tissue Culture (PTC).I also explained that the pH of the prepared MS medium should be maintained between 5.8 and 5.9.Additionally, I demonstrated how to adjust the pH using NaOH or HCl if it becomes higher or lower than the required range.

7 Jan 2026

Today, I taught Gayatri, Jyotsna, and Niraj the plant tissue culture technique for sweet potato.

9 Jan 2026

I taught Niraj Masirkar and Gayatri how to prepare the H₂S kit for water testing. During the training, I explained the complete procedure, including preparation of the medium, use of Whatman filter paper No. 41 (strip size 8 × 2.5 cm), autoclaving of all materials used for kit preparation, rolling of the strips, and placing the strips into bottles under sterile conditions.

18 Jan 2026

Teaching soil testing to 8 students who had come from a Educational trust in Bhor village.

Activity No.3

BARC Culture Replating And Multiplication

Date : 22 March 2026

Definition :

BARC culture is a set of scientific methods used at BARC to grow plant or microbial cells in sterile nutrient media inside laboratory conditions to study their growth, regeneration, or biochemical properties.

Beneficial bacterial BARC cultures are laboratory-grown bacterial strains used for agricultural and biotechnological applications to enhance soil fertility, plant growth, and biocontrol activity.

Materials :

Conical FlaskAutoclave
Petri PlateOven
SpreaderLaminar air flow
WireloopIncubator
MicropipetteBeaker

Chemicals Use

BARC Culture
PDA( Potato Dextrose Agar) / NA( Nutrients Agar )
NB( Nutrients broth )

Replating of BARC Culture

1. First, take 50 ml of distilled water in a clean conical flask.Add 3.9 g of PDA (Potato Dextrose Agar) powder to it and mix well.After the powder dissolves completely, make up the volume to 100 ml using distilled water.

2. Then, autoclave the PDA solution, Petri plates, and spreader.

3. At the same time, clean Laminar air flow using aceton / 70% ethanol and ON UV light for 15-20 min.after 20 min OFF UV light and and ON blower and day light.

4. After autoclaving, the PDA solution, Petri plates, and spreader are brought into the laminar airflow, and the PDA solution is poured into the Petri plates.

5. After the media solidifies, 0.2 ml of BARC culture is taken using a micropipette, added onto the Petri plate media, and evenly spread using a spreader.

6. Follow same protocol for all the petri plates with same concentration of BARC culture and spread equally by using spreader.

7. Packed petri plates with parafilm. And kept it in incubator at 37 C temp for 4 – 5 days.

8. After 5 days, bacterial growth appears on the plate.

Preparation of Nutrient Broth media:

  1. First, take 250 ml of distilled water in a clean conical flask.Add 6.5gm of NB (Nutrients Broth) powder to it and mix well.After the powder dissolves completely, make up the volume to 500 ml using distilled water.

2. Prepare 500 ml NB broth and sterilized by using autoclave at 121° C temp and 15 lbps pressure for 20 min.

3. After autoclaving, the NB (Nutrient Broth) media is taken to the laminar airflow and allowed to cool.After the complete cooling of NB broth, microbial colonies from incubated plates are suspended into the broth with the help of wire loop.

4. After suspension this broth is kept on orbital shaker for multiplication of bacteria at 125 – 130 rpm for 5 days.

5. Kept NB on orbital shaker for microorganisms growth.

6. After a 5-day period, prepare a mixture by adding 200gm of jaggery to 20 liters of tap water in a container. Then, transfer 500 ml of NB (nutrient broth) containing microbial colonies into the mixture. Stir gently 2-3 times and incubate for an additional 5 days.

Result

Mass multiplication of BARC culture has been successfully accomplished.

Activity No.4

making bacterial Art Using Ager Plate

Date : 3 March 2026

Objective : To create designs or words using bacteria on nutrient agar plates. This activity helps students understand bacterial growth, aseptic technique, and how microbes can be used in creative scientific expression.

What will you make?

  • A Bacterial Art Plate — a Petri dish showing a creative design, pattern, or letters made using pigmented bacterial cultures.
  • Materials Needed
  • Nutrient Agar medium (ready-made or prepared in lab)
  • Sterile Petri plates
  • Pigmented bacterial culture (e.g., Micrococcus luteus, Serratia marcescens, or safe classroom strains)
  • Cotton swabs or inoculating loop
  • Marker and labels
  • Sterile gloves
  • Spirit lamp or alcohol burner
  • 70% alcohol or sanitizer
  • Incubator (30–37°C) or warm corner for growth
  • Equipment
  • Autoclave or pressure cooker (for sterilization)
  • Hot plate or microwave (for melting agar)
  • Laminar air flow or clean working area
  • Wire loop holder
  • Waste disposal beaker with disinfectant

Collect Bacteria Sample

Procedure

  1. Prepare Nutrients Agar plate
  2. Take a 250 ml conical flask, clean it properly, and then wash it once with distilled water.
  3. Add 50 ml of distilled water to the conical flask, then weigh 2.8 g of nutrient agar and add it. After mixing, make up the solution to 100 ml with distilled water.
  4. Clean 5 Petri plates, then keep them in a hot air oven at 120°C. After 10 minutes, remove them from the oven, wrap them in an autoclave bag along with the conical.
  5. After wrapping the Petri plates and nutrient agar in an autoclave bag, place them in the autoclave at 121°C and 15 psi pressure for 15–20 minutes.
  6. After cleaning the laminar air flow, switch on the UV light for 15 minutes.
  7. After autoclaving, remove the nutrient agar and Petri plates, and place them in the laminar air flow with the blower and light on.
  8. Pour warm, sterile nutrient agar into Petri dishes (about 15–20 mL each).
  9. Let them cool and solidify completely ( 10-15 minutes )
  • Plan Your Design:
  • Draw your design or text on paper first to guide your art.
  • Choose 1–2 types of bacteria with different colors for contrast.
  • Inoculation (Drawing with Bacteria):
  • Work near a flame or in a clean area.
  • Dip a sterile cotton swab or loop into the bacterial culture.
  • Gently “draw” or “write” on the agar surface according to your design.
  • Do not press too hard—just touch the surface lightly.
  • Label and Incubate:
  • Close the lid and tape the plate edges.
  • Label with your name, date, and type of bacteria used.
  • Place the plate upside down in an incubator at 30–37°C for 24–48 hours.
  • Observe the Result:
  • After incubation, observe the design formed by bacterial colonies.
  • Different bacteria will produce different colors and textures.
  • Record your observations and take a photo of your bacterial art.
  • Safety Precautions
  • Always wear gloves and avoid touching bacterial cultures directly.
  • Keep food and drinks away from the lab.
  • Dispose of used plates by soaking them in disinfectant (bleach) before throwing away.
  • Wash hands thoroughly after the activity.
  • Use only safe, non-pathogenic bacterial strains approved by your teacher.

Activity No.5