MY DAILYWORK

Activity no. 1

Date 20/08/2022

Plant observation in soil and compost soil

Plant observation in a soil and compost soil…into the poly house
Used plants for observation
1.Brinjal
2.Cabbage
3.Tomato
4.Chilli
Requirements used for this experiment:
Soil, Compost,80 Polythene bags, Spade, Mattock
Used Reagents for the treatment :
Trichoderma powder,oilsoup
Date of plantation:
07 August 2022
1.This project we handwork from Kartik.

Date 21/08/2022

Holiday

Date 22/08/2022

For brinjal plant

observation

Soil	Observation	Compost soil	Observation
1 Healthy plant	Plants height is 34cm Leaves size 20cm	1 Healthyplant	Plants height is 34cm Leaves size 20 cm 3 Flowers observed
2 Healthy plant	Plants height is 20cm Leaves size 15cm	2 Disease plant	Plants height is 38cm Leaves size 25 cm 7 Flowers observed
3 Healthy plant	Plants height is 19cm Leaves size 14 cm	3 Disease plant	Plants height is 34cm Leaves size 30 cm 7 Flowers observed 1 Fruit observed
4 Healthy plant	Plants height is 20cm Leaves size 14 cm	4 Healthy plant	Plants height is 28cm Leaves size 19cm 9 Flowers observed
5 Healthy plant	Plants height is 18cm Leaves size 16 cm 3 Flowers observed	5 Disease  plant	Plants height is 29cm Leaves size  24cm 9 Flowers observed
6 Healthy plant	Plants height is 28cm Leaves size 18 cm	6 Healthy plant	Plants height is 38cm Leaves size 28 cm 4 Flowers observed
7 Healthy plant	Plants height is 20cm Leaves size 18 cm 3 Flowers observed 1 Fruit observed	7 Healthy plant	Plants height is 38cm Leaves size 30cm 3 Flowers observed 1 fruit obsreved
8 Healthy plant Replace date 24/08/2022	Plants height is 25cm Leaves size 19cm 2 Flowers observed	8 Healthy plant	Plants height is 38cm Leaves size 27 cm 10 Flowers observed
9 Healthy plant Replace date 24/08/2022	Plants height is 30cm Leaves size 24 cm 4 Flowers observed	9 Healthy plant	Plants height is 50cm Leaves size 30 cm 3 Flowers observed
10 Healthy plant Replace date 24/08/2022	Plants height is 39cm Leaves size 19cm 4 Flowers observed	10 Disease plant	Plants height is 57cm Leaves size 32 cm 2 Flowers observed

1. After the observation we see that plant growth in compost soil is high as compared to the plant growing in soil

      2.flowers are earlier observed in compost soil plants.

      3.Also fruits are earlier seen in compost soil.

      4.Disease observed in compost soil plants.

      5.0verall compost soil is best for the plantation.

Date 23/08/2022

For a cabbage plant

Observation:

Soil	Observation	Compost soil	Observation
1 Disease plant	Plants height is 8cm Leaves size 10cm	1 Disease plant	Plants height is 10cm Leaves size 14cm
2 Healthy plant	Plants height is 10cm Leaves size 14 cm	2 Healthy plant	Plants height is 19cm Leaves size 20cm
3 Healthy plant	Plants height is 14cm Leaves size 14 cm	3 Healthy plant	Plants height is 7cm Leaves size 7cm
4 Healthy plant	Plants height is 8cm Leaves size 9 cm	4 Disease plant Replace date 25/08/2022	Plants height is 7cm Leaves size 8 cm
5 Healthy plant	Plants height is 9cm Leaves size 10 cm	5 Disease plant Replace date 25/08/2022	Plants height is 9cm Leaves size 11 cm
6 Healthy plant	Plants height is 14cm Leaves size 14 cm Flowers observed	6 Healthy plant	Plants height is 15cm Leaves size 20 cm
7 Healthy plant	Plants height is 15cm Leaves size 14 cm	7 Healthy plant	Plants height is 19cm Leaves size 18 cm
8 Healthy plant	Plants height is 10cm Leaves size 9cm	8 Healthy plant	Plants height is 16cm Leaves size 14 cm
9 Disease plant Replace date 25/08/2022	Plants height is 14cm Leaves size 14 cm	9 Healthy plant	Plants height is 14cm Leaves size 14 cm
10 Healthy plant	Plants height is 14cm Leaves size 14 cm	10 Disease plant	Plants height is 14cm Leaves size 17 cm

1. After the observation we see that plant growth in compost soil is high as compared to the plant growing in soil

      2.flowers are earlier observed in compost soil plants.

      3.Also fruits are earlier seen in compost soil.

      4.Disease observed in compost soil plants. 

      5.0verall compost soil is best for the plantations. 

Date 24/08/2022

For a Tomato plant

Observation:

Soil	Observation	Compost soil	Observation
1 Healthy plant	Plants height is 50cm Leaves size 30cm 7 flowers observed. 10 Fruits observed.	1 Healthy plant	Plants height is 80cm Leaves size 42cm 5 Flowers observed 11 fruits observed
2 Healthy plant	Plants height is 50cm Leaves size 20 cm 8 Flowers observed. 6 Fruits observed.	2 Healthy plant	Plants height is 105cm Leaves size 41 cm 4 Flowers observed 10 Fruits observed.
3 Healthy plant	Plants height is 14cm Leaves size 14 cm 3 Flowers observed. 4 Fruits observed.	3 Healthy plant	Plants height is 85cm Leaves size 40 cm 15 Flowers observed 16 Fruits observed.
4 Healthy plant	Plants height is 56cm Leaves size 25cm 5 Flowers observed 7 Fruits observed.	4 Healthy plant	Plants height is 100cm Leaves size 44 cm 9 Flowers observed 9 Fruits observed
5 Healthy plant	Plants height is 40cm Leaves size 20 cm 2 Flowers observed. 4 Fruits observed.	5 Healthy plant	Plants height is 70cm Leaves size 42cm 2 Flowers observed 12 Fruits observed
6 Healthy plant	Plants height is 50cm Leaves size 30 cm 3 Flowers observed. 8 Fruits observed.	6 Healthy plant	Plants height is 85cm Leaves size 41cm 4 Flowers observed 14 Fruits observed.
7 Healthy plant	Plants height is 60cm Leaves size 34 cm	7 Healthy plant	Plants height is 100cm Leaves size 42 cm 3 Fruits observed
8 Disease plant Replace date 26/08/2022	Plants height is 50cm Leaves size 34 cm	8 Healthy plant	Plants height is 107cm Leaves size 40cm 14 Flowers observed 11 Fruits observed
9 Healthy plant	Plants height is 50cm Leaves size 34 cm	9 Healthy plant	Plants height is 104cm Leaves size 42 cm 6 Flowers observed 8 Fruits observed.
10 Disease  plant Replace date 26/08/2022	Plants height is 59cm Leaves size 35 cm	10 Healthy plant	Plants height is 107cm Leaves size 44 cm 42 Flowers observed 6 Fruits observed.

1. After the observation we see that plant growth in compost soil is high as compared to the plant growing in soil

      2.flower are earlier observed in compost soil plants.

      3.Also fruits are earlier seen in compost soil.

      4.Diseas observed in compost soil plants. 

      5.0verall compost soil is best for plantation. 

Date 25/08/2022

For a Chilli plant

observation:

Soil	Observation	Compost soil	Observation
1 Disease plant Replace date 27/08/2022	Plants height is 20cm Leaves size 14 cm	1 Healthy plant	Plants height is 24m Leaves size 17 cm
2 Healthy plant	Plants height is 54cm Leaves size 30 cm	2 Disease plant	Plants height is 65cm Leaves size 24 cm 10 Flowers observed
3 Disease plant Replace date 27/08/2022	Plants height is 14cm Leaves size 6 cm	3 Disease plant	Plants height is 21cm Leaves size 8 cm
4 Healthy plant	Plants height is 15cm Leaves size 10 cm	4 Healthy plant	Plants height is 22cm Leaves size 12cm
5 Healthy plant	Plants height is 11cm Leaves size 8 cm	5 Disease plant Replace date 27/08/2022	Plants height is 11cm Leaves size 5 cm
6 Healthy plant	Plants height is 14cm Leaves size 10 cm 6 Flowers observed 5 Fruits observed	6 Healthy plant	Plants height is 25cm Leaves size 23 cm
7 Disease plant Replace date 27/08/2022	Plants height is 35cm Leaves size 14 cm	7 Healthy plant	Plants height is 60cm Leaves size 23cm 8 Flowers observed
8 Healthy plant	Plants height is 16cm Leaves size 14 cm	8 Healthy plant	Plants height is 56cm Leaves size 23 cm 8 Flowers observed
9 Healthy plant	Plants height is 44cm Leaves size 24 cm	9 Healthy plant	Plants height is 62cm Leaves size 23 cm 11 Flowers observed
10 Healthy plant	Plants height is 50cm Leaves size 23 cm 3 Flowers observed	10 Disease plant	Plants height is 60cm Leaves size 19cm 4 Flowers observed

1. After the observation we see that plant growth in compost soil is high as compared to the plant growing in soil

      2.flower are earlier observed in compost soil plants.

      3.Also fruits are earlier seen in compost soil.

      4.Diseas observed in compost soil plants.

      5.0verall compost soil is best for the plantation.

Activity no.2

Date 26/08/2022

Geranium planting

1.There are 70 Geranium plants we growing in front of fab lab in Ashram.

2.Firstly we make the pits in soil at 20 cm distance.

3.After that plant growing.

4.Add soil and compost.

5.Every day at the afternoon we gives 1L water each plant.

6.Plants growth is increasing day by day .

Geranium plant used for the making Oil,Perfume and soup industries.

It helpful for the pollination.

Due to there fragrance birds attracted early.

Activity no.3

Date 27/08/2022

Fish weighting

There are four tanks in which we keeping koi fish.

1)first tank ( Gives feed & fermented Azolla )

It is second time of fish weighting.

Firstly remove all water from the tank.

Catch the all fishes in a tray.

Weight it on a weight box

It is about 85kg

The weight is increasing after the one month.

2) Second tank(Gives only Feed )

It also second time of fish weighting.

Firstly remove all water from the tank.

Catch the all fishes in a tray.

Weight it on a weight box.

It is about 67 kg

The weight is at the same do not changes.

3)third tank ( Gives feed & boil azolla)

It also second time of fish weighting.

Firstly remove all water from the tank.

Catch the all fishes in a tray.

Weight it on a weight box.

It is about 90 kg

The weight is at the same do not changes.

4)Fourth tank (Gives feed and fermented azolla)

It also second time of fish weighting.

Firstly remove all water from the tank.

Catch the all fishes in a tray.

Weight it on a weight box.

It is about 110 kg

The weight is at the same do not changes.

At the end we clearly observed that the weight of fish increases in that tank we gives feed and boil azolla as well as fermented azolla.

In second tank we gives feed but not azolla therefor the weight of fish do not well increases.

Date 28/08/2022

Holiday

Activity no.4

Date 29/08/2022

Azolla cultivation

We cultivated azolla from 11 water beds.

these azolla weight is 480 kg.

cultivated azolla dry under the sunlight.

These dry azolla used as feed for the cattle,poultry,fishes and husbundry.

Activity no. 5

30/08/2022

Soil testing

Soil testing element detection (N,P,K),pH test,TDS,EC.soil testing provides valuabale information on pH and plant available nutrient.

soil Testing provides valuable information on pH and plant available nutrients.

essential elements of soil testing that are Nitrogen, Phosphorus, potassium , etc.

all elements in soil that are.

Apparatus used for soil testing:

weighting balance, measuring Cylinder,Glass beaker, various reagents used for soil testing, test tube , funnel, filter paper, tissue paper, cotton, butter paper.

soil testing

test for Nitrogen

test for Phosphorus

test for potassium

test for pH.

Result.

In a soil we observed N, P, K elements are presents in essential amount and pH

In this experiment we learn how to detect elements from a given soil sample.

soil Testing elements

Activity no. 6

Date 31/08/2022

Moringa cuttings plantation

We Growing Moringa plants cuttings in red soil and black soil.

to observe how to cuttings growing in different soils

Activity no. 7

Date31/08/2022

Inside the poly house 12 plants in red soil and 12 plants in black soil

Outside the poly house 12 plants in red soil and 12 plants in black soil growing for observation.

Geranium plantation

There are 24 geranium plants growing in red soil

12 plants kept in poly house and 12 are kept outside the poly house for observation

24 geranium plants growing in black soil

12 plants kept in poly house and 12 are kept outside the poly house for observation

Activity no.8

01/01/2022

1)

lab cleaning,lab rearrangement

lab were cleaned by using Dettol and ethanol

rearrange all the instruments.

All glassware bottle,tubes and Petri plates were cleaned and dried using Hot Air oven and Autoclaved all of them.

2)

Distilled water collection and sterilizaion

today we learn water distilation process that relies on evaporation to purify water.

practically learn water distillation experiment.

Date 02/09/2022

Holiday

Activity no.9

Date 03/09/2022

Preparation of H2S kit for water testin

Media preparation for 300 H2s kit strip

Preparation of 50 strips

Use Whatman filter paper no.41 for the making strips of H2S water testing kit.

Cut the paper in size of 8*2.5 cm for making 300 strips.

Dip all strips in given media which is mention in Step-1Use Hot Air oven to dry strips at 50 degree Celsius for 40 min. After that use Laminar Air flow for sterilizing the strips.

Take 30 ml new 300 plastic bottles Sterile the bottles using Laminar Air Flow for 1 hrs.

It helps to prevent Bacterial contamination.

Making the H2S bottles with strips which is coated by H2S media Getting precautions for avoid the contamination70% ethanol sterile gloves.

Use forceps for put strips into the bottles.

Bottles caps should be tight.

All process are done under the UV light of Laminar Air Flow.

Date 04/08/2022

Holiday

Activity no.10

Date 05/09/2022

Estimation of carbon from soil by wilky black method

Introduction:- Estimates of organic carbon are used to assess the amount of organic matter in soil . The method measures the amount of organic carbon in plant and animal remains including soil humus . 

Aim :- To estimate organic carbon from soil sample by walkey black method

Equipment:–

1. 500-mL Erlenmeyer flasks.

2. 10-mL pipette.

3. 50-mL burette

.4. Analytical balance.

5. Incandescent lamp.

Reagents:

1. H3PO4, 85%.

2. H2SO4, concentrated (96%)

3. NaCl , solid

.4. Standard 0.167M K2Cr2O7:- Dissolve 4.904 g of dried (105oC) K2Cr2O7 in water and dilute to 100ml.

5. 0.5M Fe2+ solution: Dissolve 49 g of Fe(NH4)2(SO4)•6H2O in 250 mL of water containing 5 mL of concentrated H2SO4 and dilute to 250 ml. The Fe2+ in this solution oxidizes slowly on exposure to air so it must be standardized against the dichromate.

6. Ferroine indicator

Procedure:

1. Weigh 1 g dried soil and transfer to a 500 mL Erlenmeyer flask. 

2. Add 10 ml K2Cr2O7 solution in it.

3. Add 20 mL of concentrated H2SO4 by means of a dispenser and swirl gently to mix of the flask out of the solution.

4. Allow to stand for 30 minutes. The flasks should be placed on an insulation pad during this time to avoid rapid heat loss.

5. Dilute the suspension with about 200 mL of water to provide a clearer suspension for viewing the endpoint.

6. Add 10 mL of 85% H3PO4 and add  0.2 g of NaCl. The H3PO4 and NaCl is added to complex Fe3+ which would interfere with the titration endpoint.

7. Add 10 drops of ferroin indicator.

8. Titrate with 0.5 M Fe2+ to a burgundy endpoint. 

9. Run a reagent blank using the above procedure without soil. The blank is used to standardize the Fe2+ solution daily.

Calculations :-   

 For organic carbon=

C = (B-S) x M of Fe2+ x 12 x 100g soil / 4000

Where:   

 B  = mL of Fe2+ solution used to titrate blank        

S  =  mL of Fe2+ solution used to titrate sample

/4000 = Milliequivalent weight of C in

carbon= C = (B-S) x         B  = mL of Fe2  

Activity no.11

Date 06/09/2022

Turmeric plantaion

Today we growing turmeric plants backside of soil lab .

Firstly plants remove from pots

Then plants grow in soil arrange in line .

watering that plants and observed their growth.

Activity no.12

Date 07/09/2022

Learn Auto clave, Laminar air flow, Oven dryer working

1) Autoclave 

Aim: To study Autoclave principal, working and uses.

Principal:The basic principle of steam sterilization, as accomplished in an autoclave, is to expose each item to direct steam contact at the required temperature and pressure for the specified time. Thus, there are four parameters of steam sterilization: steam, pressure, temperature, and time.

Working:

A sufficient quantity of water is poured into the chamber and the level of water is adjusted in such a way that it is slightly touching to the bottom of the perforated chamber. The material is packed in the perforated chamber. The lid is then closed with the screw and the autoclave is switched on. Initially, discharge tap is kept open and safety valve is adjusted to required pressure. As the temperature of water increases, it starts to vaporise and result in the formation of stem. This steam removes the air from autoclave which is indicated by formation of air bubble at discharge valve. When air bubbles stop emitting from discharge tap, it indicates that complete air has been removed out. At this stage the discharge valve is closed. Once steam pressure reaches to desired value, safety valve is set open. From this point subject is hold for time as specified in table 1. After holding time autoclave is switched off and allows cooling. Then lid is opened and sterilized materials are taken out.

Applications of Autoclave:

1. Autoclaves are widely used in microbiology, veterinary science, mycology etc.

2. It is used to sterilize wide range of material including but not limited to laboratory glasswares, laboratory equipments and instruments, surgical material including needles, seizures, heat-stable hand gloves, containers, and closures etc.

3. Autoclave is most commonly involved in the sterilization of biological media

2)Laminar air flow:

Aim: To study laminar air flow principle, working and uses.

Principal:

Laminar airflow, also known as laminar air flow (LAF), is a device designed to prevent the equipment and working environment from particles. Laminar airflow units create particle-free working environments by sucking air through a filtration system and exhausting it across a work surface in a laminar air stream.

Working:

Laminar air flow unit works by the use of in-flow laminar air drawn through one or more HEPA filters, designed to create a particle-free working environment and provide product protection. Air is taken through a filtration system and then exhausted across the work surface as part of the laminar flow process.

Commonly, the filtration system comprises a pre-filter and a HEPA filter.

First, the air goes through the pre-filter (panel filter). Next, the fan blows the air towards the HEPA filter. HEPA filter captures the dust, bacteria, and airborne particles. Therefore, the HEPA filtered air is clean and particle-free.

Application:

Laminar flow cabinets are used in laboratories for contamination sensitive processes like plant tissue culture.

Other laboratories processes like media plate preparation and culture of organisms can be performed inside the cabinet.

Operations of particle sensitive electronic devices are performed inside the cabinet.

In the pharmaceutical industries, drug preparation techniques are also performed inside the cabinet to ensure a particulate-free environment during the operations.

Laminar flow cabinets can be made tailor-made for some specialized works and can also be used for general lab techniques in the microbiological as well as the industrial sectors.

3) Oven dryer:

Aim:To study oven dryer principle,working and uses.

Principle & working:

Sterilization by dry heat is performed by conduction. The temperature is consumed by the surface of the objects, then moves towards the core of the object, coating by coating. The whole object will ultimately attain the temperature needed for sterilization to take place.

Dry heat causes most of the injury by oxidizing particles. The primary cell components are damaged and the organism dies. The temperature is kept for about an hour to eliminate the most ambitious of the resistant spores.

Uses:

• No need to water to sterilize the material.
• Not much pressure is created like autoclave which creates it easy to manage and also makes it safer to work with.
• In a laboratory environment, it is more fitting to use as compared to other sterilizers.
• Hot air oven is much smaller in size as compared to autoclaves and also more effective.
• A hot air oven can be more speedy than an autoclave and higher temperature can be achieved as compared to other means.
• The operating procedure is simple as compared to other sterilization methods.
• Its price is low as compared to autoclave. 

Activity no.13

Date 08/08/2022

Humidity & temperture

Everyday We checked Humidity & Temperature inside and outside of the poly house.

If temperature is observed high then we sprying water for maintain temperature & humidity.

Weekly observation of Temperature & Humidity inside and outside of Poly house.

Weekly observation of temperature & Humidity Inside the Poly house and outside the poly house

Activity No.14

Date 09/09/20

Geranium Plant tissue culture

Today we learn Plant Tissue Culture from Akshada and Ayesha .

We select Three plants for tissue culture.

1.Geranium.

2.Shivsuman.

3.Tobbaco.

From all the three plants we select stem or shoot for the tissue culture.

Then we cut stem into small pieces.

We use Agar which is already prepared.

We Prepared following Plant tissue culture :

1.Geranium plant

In 4 Bottles and 4 Test tubes

2.Shivsuman plant

In 4 Bottles and 4 Test tubes

3.Tobbaco plant

In 10 Bottles and 4 Test tubes

Activity no.15

Date 10/09/2022

lab cleaning & Ashrsm tour with tourist.

Today we cleaned all lab and lab apparatus.

Miss.kulkarni and their team came from Nashik to visit the Vigyan ashram Pabal.

The whole day their team stay here and learn all information about the Ashram.

Mr. Mahendra sir guide them. They told all information and rules and all structure of vigyan ashram.

After all they visited IBt school.

Date 11/09/2022

Holiday

Activity No.16

Date 12/09/2022

Azolla removeing from five beds

Today we remove azoll from all the tanks and dry it into the sunlight.

Activity No.17

Date 13/08/2022

Blood Group Testing

Today we learn blood group testing in food lab.

In food lab DBRT students learn blood group testing practical, that time we went there.

We studied which antigen used for the blood group testing like as Anti-A ,Anti-B,Anti-D.

For the blood group testing we used my blood drops.

At the end we detect AB+ blood group.

Activity no.18

MS Media Preparation

Date 14/08/2022

Introduction:
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. 

Requirements :

For 500 ml Media preparation –

Stock 1	12.5 ml
Stock 2	0.5 ml
Stock 3	2 ml
Stock 4	0.5 ml
Mynosyitol	50 mg
Sucrose	15 gm
Agar	4 gm

Procedure:

1. Take all stock solution in flask as per measurement .

2. Add 50 mg mynosyitol .

3. Add 15 gm sucrose .

4. Add distill water Mix it well .

5. check Ph of Media if Ph is less than 5.8 then maintain it by using NaOH .

6. Add 4 gm Agar .

7. Now heat the media on flame till the agar gets completely dissolved .

8. Now pour the media into bottles and autoclave it .

Conclusion :

This media is use full for tissue culture plants .

Activity no.19

Date 15/09/2022

Geranium Farming

Introduction:

We Visited to a Geranium plant which belong to Khaire and is Known as Khaire Farms.

Its Located on Mhase BK Maharashtra 412118

https://goo.gl/maps/mHJZCaCWgZAWX4Rp9

About Geranium:-

Geranium essential oil is derived by steam distillation of the leaves of Pelargonium graveolens, a plant species native to South Africa. According to folklore, it was used for a wide range of health conditions.

Geranium oil is grown in many regions, including Europe and Asia. There are many varieties and strains of the pink flower with a fresh, floral fragrance. Each variety differs in scent, but is near-identical in terms of composition, benefits, and uses.

Geranium essential oil can be diluted with a such as sesame oil, and used topically on the skin. You can use it as a spot treatment for acne or itchy skin, or as a massage oil.

Some carrier oils may cause an allergic reaction when applied to the skin. Prior to using, do a patch test on a small area to make sure it doesn’t cause a reaction.

Geranium oil benefits:-

Because geranium oil is a natural anti-oxidant and anti-inflammatory oil, it actively boosts skin’s health and natural glow. By helping skin stay conditioned, and soothing irritated or breakout prone skin, it fights against skin affected by harsh weather conditions or prone to acne

Information about Geranium farming

Name of Plant :- Geranium

Land Holding:- 18 acre

Number of plants in acre:- 10000 plants

Fertilizers:- compost fertilizer

Yield:- 10 tonne per acre ( plant should be dry)

Amount of oil extracted for 1 acre:- 20 lit

Amount per lit:- Rs11500

Users:- Mumbai company

Remaining Product:- converted to compost fertilizer

Time Taken:- 3 Month

Irrigation method:- Drip

Plantation peroid:- June- Aug , Sep -Oct ( very less rain required )

Plant prize per plant :- 3 to 4 Rs

Media use:- cocopit

Row to Row distance:- 3 feet and Plant to plant distance:- 1.25 feet

Drip:- 3 to 4 for 2 hrs

Water Supply:- River, farm pond

Distillation Maching porduction :- 3 tonne :-6 lit

Drip Dicharge :- 20 mm Drip :- 4 lit water to each plant

Manpower :- 5-6 Labor

They bring water from Ghod & Kokdi river through pipeline from a distance of half a kilometer.

2 crore litre Farmpond

They can stored river water in their Farmpond, Capacity of this farmpond is 2 crore litres. They have 40 feet depth.

They have installed Geranium Distillation Plant from Rubicon Watering engineering company.

Component of Distillation Plant: Boiler, Blower, Condenser, Steam Flow pipe, 500kg capacity Two tanks, etc.

Process: Firstly, To collect the geranium cutting leaves in tank. To start the boil water and convert water into steam. steam pass through the steam pipe connected to the 1st tank, they gives steam to tank and they increases the temperature of tank then To separate the oil and leaves. oil and water steam goes to condenser and after all we have to separate out Geranium oil and remaning steam water is called as hydrosol.

Conclusion : 500kg of geranium plants yield 1 litre of geranium oil and 4 litre of hydrosol.

I got to learn so many new things, I didn`t knew what geranium was, it was great experance.

Activity no.20

Date 16/09/2022

Peanut-Chikki

Roast Peanuts

1. Heat a thick-bottom heavy kadai or pan and add 1 cup of peanuts.

2. Roast the peanuts on a low to medium heat and stir from time to time until they become crunchy.

3. Roast until black spots appear on the peanuts, taste them and make sure they have some crunch. If the peanuts are still raw then roast them for longer.

4. Once the peanuts are roasted add them to a separate plate or tray and leave them to cool down.

5. Grease the back of a metal baking tray, steel plate, or marble board with oil or ghee. You can also brush a parchment paper kept on a sturdy surface with oil or ghee.

6. Once the peanuts cool down, rub them between your palms and peel off the husks.

7. Add peanuts to a sieve or winnow with large perforations and sift to remove husks. Remove husked peanuts and set them aside.

Make Jaggery Syrup

8. jaggery block instead. Chop it finely and measure the chopped jaggery in a ½ cup measuring cup

9. Add 2 tablespoons of water.

10. Heat the kadai (wok) or pan on a low flame. Then, use a spatula or spoon to stir the jaggery syrup until the jaggery dissolves.

11. Continue to stir the mixture continuously.

12. The jaggery syrup should begin to thicken. Continue to stir the syrup on a low flame.

13. Add cold water to a bowl and keep it close by. Then add a few drops of jaggery syrup to the water to check the consistency of the jaggery syrup as it cooks.

14. The syrup will thicken and begin to bubble.

15. Continue to cook jaggery syrup on low heat and stir often.

16. Cook the mixture until it starts to thicken. The color of the syrup will change and darken. At this point, the syrup should be stirred non-stop to prevent burning.

17. Eventually the syrup should resemble molten liquid mass.

18. Check the consistency by adding a few drops of the jaggery syrup to the cold water. The jaggery syrup should be firm, brittle, and snap easily. This is referred to as the hard ball stage.

Make Peanut-Chikki

19. Once the jaggery syrup is the right consistency, add the peanuts quickly.

20. Mix the jaggery syrup and peanuts until well combined.

21. Switch off the heat and quickly pour the chikki mixture onto a greased plate or marble board.

22. Place foil, butter paper, or parchment paper on the chikki mixture. Use a rolling pin to flatten the chikki mixture into one even layer.

23. After rolling the chikki mixture, remove the parchment paper.

24. After removing the paper, carefully cut the chikki into equal-sized squares. 

25. Leave the chopped chikki to cool at room temperature.

26. Once the peanut chikki has cooled down break and serve. If you want to serve the chikki at a later time then store it in an airtight jar at room temperature.

Activity no.21

Date 17/09/2022

Pesticides spraying On Plants

Today we spraying Pesticides and required nutrients.

Calcium nitrate,

Date 18/09/2022

Holiday

Activity no.22

Date 19/09/2022

Watering all plant in Polyhouse

Today We watering all the plants in polyhouse

Activity no.23

Date 20/09/2022

Media Preparation & Pouring into Bottles

Introduction:
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. 

Make Culture + Sucrose media

Requirements :

For 500 ml Media preparation –

Stock 1	12.5 ml
Stock 2	0.5 ml
Stock 3	2 ml
Stock 4	0.5 ml
Mynosyitol	50 mg
Sucrose	15 gm
Agar	4 gm

Procedure:

1. Take all stock solution in flask as per measurement .

2. Add 50 mg mynosyitol .

3. Add 15 gm sucrose .

4. Add Culture Mix it well .

5. check Ph of Media if Ph is less than 5.8 then maintain it by using NaOH .

6. Add 4 gm Agar .

7. Now heat the media on flame till the agar gets completely dissolved .

8. Now pour the media into bottles and autoclave it .

Activity no.24

Date 21/09/2022

Media Preparation & Pouring Into Testtubes

Introduction:
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. 

Make media instead of distiled water use culture

Requirements :

For 500 ml Media preparation –

Stock 1	12.5 ml
Stock 2	0.5 ml
Stock 3	2 ml
Stock 4	0.5 ml
Mynosyitol	50 mg
Sucrose	15 gm
Agar	4 gm

Procedure:

1. Take all stock solution in flask as per measurement .

2. Add 50 mg mynosyitol .

3. Add Culture Mix it well .

4. check Ph of Media if Ph is less than 5.8 then maintain it by using NaOH .

5. Add 4 gm Agar .

6. Now heat the media on flame till the agar gets completely dissolved .

7. Now pour the media into Testtubes and autoclave it.

Activity no.25

Date 22/09/2022

Potato Dextrose Agar Prepartion and pour into petriplates

Date 23/09/2022

Holiday

Activity no.26

Date 24 /09/2022

Azolla removing and watering all plants

Today we remove azolla and give water all the plants

Date 25/09/2022

Holiday

Activity no.27

Date 28/09/2022

Preperation of 0.1 molar NaOH solution

Procedure:

1.take about 100ml distilled water in a cleaned and dried 1000 ml volumetric flask.

2.Add adout 4.2 gm of sodium hydroxide with continues stirring.

3.Add more about 700 ml of Distilled water ,mix and allow to cool to room temperature.

4.Make the volume 1000ml with distilled water.Mix solution thoroughly.

5.Keep the Solution for at least an hour and then carry out the standardization.