DESIGN OF EXPERIMENT FOR GROWTH OF AZOLLA

Setup :- 1

10-06-2022

Project on experiment for growth of azolla.

INTRODUCTION

Azolla is a branched free-floating aquatic fern, and it rapidly grows on the water’s surface. Besides its utilization as a biofertilizer on a variety of crops, Azolla can be used as an animal feed, a human food, a medicine, and a water purifier. Azolla grows best in full to partial shade (25-50% of full sunlight). Growth decreases quickly under heavy shade (lower than 1500 lux) and more than 50% of full sunlight reduces photosynthesis. The optimum relative humidity for Azolla growth is between 85 to 90 %.

MATERIAL AND FERTILIZER

1.20 tays

2.soil

3.water

4.Fertilizers

PSB-200mg

KH₂PO₄-435mg

MGSO₄-3mg

FESO₄-15mg

FENO₃-40mg

PROCEDURE

• Take 20 trays of same size .add 10 gm soil in each tray

Sr no	Fertilizers	Set-1	Set- 2
1	SOIL	10gm	10gm
2	PSB	200mg	1000mg
3	KH2PO4	435mg	2.175gm
4	MgSO4	3mg	15mg
5	FeSO4	40mg	75mg
6	FeNO3	40mg	200mg

we were arranged all these 20 trays in two sets as following

Setup :- 2

17/06/2022

OBSERVATION –

23/06/2022

Set ;- A

	Tray weight	Wet weight	Dry weight
1)	0.770 gm	20.586 gm	0.918 gm
2)	0.865 gm	17.480 gm	0.602 gm
3)	0.995 gm	27.718 gm	1.511 gm
4)	0.930 gm	21.690 gm	0.810 gm
5)	0.975 gm	25.858 gm	1.429 gm
6)	0.860 gm	19.096 gm	0.538 gm
7)	0.870 gm	20.854 gm	0.977 gm
8)	0.889 gm	17.564 gm	0.716 gm
9)	0.953 gm	17.860 gm	0.920 gm
10)	0.974 gm	15.399 gm	0.563 gm

Set ;- B

	Tray weight	Wet weight	Dry weight
i)	0.865 gm	11.887 gm	0.448 gm
ii)	0.920 gm	12.490 gm	0.540 gm
iii)	0.894 gm	13.847 gm	0.762 gm
iv)	0.852 gm	13.678 gm	0.714 gm
v)	0.858 gm	12.926 gm	0.430 gm
vi)	0.915 gm	19.144 gm	0.736 gm
vii)	0.682 gm	14.104 gm	0.780 gm
viii)	0.680 gm	18.350 gm	1.500 gm
ix)	0.712 gm	17.110 gm	0.892 gm
x)	0.675 gm	12.943 gm	0.762 gm

CONCIUSION –

In this experiment , we observed that trey no. 6 which contains PSB+MgSO4+FeSO4 have a double growth rate of Azolla within 6 days. This combination of fertilizers can be used further for culturing of Azolla.