PREPARE 50 H2S WATER TESTING KIT

STEP-1 (23/09/2021)

Media preparation for 50 H2S kit strip

Name of chemicalsQuantity in gm/ml
Bacteriological Grade Peptone20 gm
Dipotassium Hydrogen Phosphate1.5 gm
Ferric Ammonium Citrate 0.75 gm
Sodium Thiosulphate1.0 gm
L-cysteine HCl0.125 gm
Distilled water50 ml
Teepol1.0 ml

STEP-2 (24/09/2021)

Preparation of 50 strips

  • Use Whatman filter paper no-41 for the making strips of H2S Water testing kit.
  • Cut the paper in size of 8*2.5 cm for making 50 strips.
  • Dip all strips in given media which is mention in Step-1
  • Use Hot Air oven to dry strips at 50 degree Celsius for 40 min
  • After that use Laminar Air flow for sterilizing the strips.

STEP -3 (26/09/2021)

  • Take 30 ml new 50 plastic bottles
  • Sterile the bottles using Laminar Air Flow for 1 hrs.
  • It helps to prevent Bacterial contamination.

STEP -4 (26/09/2021)

Making the H2S bottles with strips which is coated by H2S media

Getting precautions for avoid the contamination

  • 70% Ethanol
  • sterile gloves.
  • Use forceps for put strips into the bottles.
  • Bottles caps should be tight.

All process are done under the UV light of Laminar Air Flow.

STEP-5 (27/09/2021)

Packaging

STEP-6

Waiting for results and feedback……..