PREPARE 50 H2S WATER TESTING KIT
STEP-1 (23/09/2021)
Media preparation for 50 H2S kit strip
Name of chemicals | Quantity in gm/ml |
Bacteriological Grade Peptone | 20 gm |
Dipotassium Hydrogen Phosphate | 1.5 gm |
Ferric Ammonium Citrate | 0.75 gm |
Sodium Thiosulphate | 1.0 gm |
L-cysteine HCl | 0.125 gm |
Distilled water | 50 ml |
Teepol | 1.0 ml |
STEP-2 (24/09/2021)
Preparation of 50 strips
- Use Whatman filter paper no-41 for the making strips of H2S Water testing kit.
- Cut the paper in size of 8*2.5 cm for making 50 strips.
- Dip all strips in given media which is mention in Step-1
- Use Hot Air oven to dry strips at 50 degree Celsius for 40 min
- After that use Laminar Air flow for sterilizing the strips.
STEP -3 (26/09/2021)
- Take 30 ml new 50 plastic bottles
- Sterile the bottles using Laminar Air Flow for 1 hrs.
- It helps to prevent Bacterial contamination.
![](http://vadic.vigyanashram.blog/wp-content/uploads/2021/09/WhatsApp-Image-2021-09-27-at-10.41.38-PM-1-1-1024x471.jpeg)
STEP -4 (26/09/2021)
Making the H2S bottles with strips which is coated by H2S media
Getting precautions for avoid the contamination
- 70% Ethanol
- sterile gloves.
- Use forceps for put strips into the bottles.
- Bottles caps should be tight.
All process are done under the UV light of Laminar Air Flow.
![](http://vadic.vigyanashram.blog/wp-content/uploads/2021/09/WhatsApp-Image-2021-09-29-at-10.45.19-AM-1024x470.jpeg)
![](http://vadic.vigyanashram.blog/wp-content/uploads/2021/09/WhatsApp-Image-2021-09-21-at-5.28.13-PM-1-1024x470.jpeg)
STEP-5 (27/09/2021)
Packaging
![](http://vadic.vigyanashram.blog/wp-content/uploads/2021/09/WhatsApp-Image-2021-09-27-at-10.07.55-PM-1024x768.jpeg)
![](http://vadic.vigyanashram.blog/wp-content/uploads/2021/09/WhatsApp-Image-2021-09-28-at-9.39.04-PM-1024x467.jpeg)
STEP-6
Waiting for results and feedback……..