MS media preparation

Introduction :

Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture.

Requirements :

For 1 lit Media preparation –Stock 1 25mlStock 2 1 mlStock 3 4mlStock 4 1 mlMynosyitol 100 mgSucrose 30 gmAgar 8 gm

Procedure:

1. Take all stock solution in flask as per measurement .

2. Add 100mg mynosyitol .

3. Add 3o gm sucrose .

4. Add distill water Mix it well .

5. check Ph of Media if Ph is less

than 5.8 then maintain it by using NaOH .

6. Add 8 gm Agar .

7. Now heat the media on flame till the agar gets completely dissolved .

8. Now pour the media into bottles and autoclave it .