AIM:Mass multipliaction of BARC culture.
REQUIREMENTS:
For Plating of BARC culture:Available BARC Culture, PDA and NA Media ,Petri plates,Spreader,Microtips and micropipette,Autoclave,Incubator Laminar, Beaker.
For Plating of BARC culture
9/7/24
- Prepared PDA and NA media .
- Autoclave media,petri plates,distilled water,test tubes,spreader,microtips at 121C temp and 15 lbp pressure for 20 min.
- For Maintaining asceptic condition : At the same time, clean LAF(laminar air flow) using aceton / 80% ethanol and ON UV light for 15-20 min.after 20 min OFF UV light and and ON blower and day light.
- Then all remaining procedure carried out under asceptic conditions.After sterilization, media poured into petri plates
- After solidification of media, add 0.2 ml of aliquot of BARC from test tube on petri plate. And spread equally on whole petri plate.
- Follow same protocol for all the petri plates with same concentration of BARC culture and spread equally by using spreader.
- Packed petri plates with parafilm. And kept it in incubator at 37 C temp for 4/5 days.
b.kept petri plate in incubator for microorganisms growth
After incubation of 5 days. Growth of micro organisms has been completed.
Preparation of Nutrient Broth media:
16/7/24
Prepare 500 ml NB broth and sterilized by using autoclave at 121° C temp and 15 lbps pressure for 20 min.
In aseptic condition after the complete cooling of NB broth, microbial colonies from incubated plates are suspended into the broth with the help of wire loop.
After suspension this broth is kept on orbital shaker for multiplication of bacteria at 125 rpm for 5 days.
a.kept NB on orbital shaker for microorganisms growth
21/7/24
After a 5-day period, prepare a mixture by adding 1 kg of jaggery to 50 liters of tap water in a container. Then, transfer 500 ml of NB (nutrient broth) containing microbial colonies into the mixture. Stir gently 2-3 times and incubate for an additional 5 days.”
Result and Conclusion:
Mass multiplication of BARC culture has been successfully accomplished.
