Definition :
BARC culture is a set of scientific methods used at BARC to grow plant or microbial cells in sterile nutrient media inside laboratory conditions to study their growth, regeneration, or biochemical properties.
Beneficial bacterial BARC cultures are laboratory-grown bacterial strains used for agricultural and biotechnological applications to enhance soil fertility, plant growth, and biocontrol activity.
Materials :
| Conical Flask | Autoclave |
| Petri Plate | Oven |
| Spreader | Laminar air flow |
| Wireloop | Incubator |
| Micropipette | Beaker |
Chemicals Use
| BARC Culture |
| PDA( Potato Dextrose Agar) / NA( Nutrients Agar ) |
| NB( Nutrients broth ) |
Replating of BARC Culture
1. First, take 50 ml of distilled water in a clean conical flask.Add 3.9 g of PDA (Potato Dextrose Agar) powder to it and mix well.After the powder dissolves completely, make up the volume to 100 ml using distilled water.
2. Then, autoclave the PDA solution, Petri plates, and spreader.
3. At the same time, clean Laminar air flow using aceton / 70% ethanol and ON UV light for 15-20 min.after 20 min OFF UV light and and ON blower and day light.
4. After autoclaving, the PDA solution, Petri plates, and spreader are brought into the laminar airflow, and the PDA solution is poured into the Petri plates.
5. After the media solidifies, 0.2 ml of BARC culture is taken using a micropipette, added onto the Petri plate media, and evenly spread using a spreader.
6. Follow same protocol for all the petri plates with same concentration of BARC culture and spread equally by using spreader.
7. Packed petri plates with parafilm. And kept it in incubator at 37 C temp for 4 – 5 days.
8. After 5 days, bacterial growth appears on the plate.
Preparation of Nutrient Broth media:
- First, take 250 ml of distilled water in a clean conical flask.Add 6.5gm of NB (Nutrients Broth) powder to it and mix well.After the powder dissolves completely, make up the volume to 500 ml using distilled water.
2. Prepare 500 ml NB broth and sterilized by using autoclave at 121° C temp and 15 lbps pressure for 20 min.
3. After autoclaving, the NB (Nutrient Broth) media is taken to the laminar airflow and allowed to cool.After the complete cooling of NB broth, microbial colonies from incubated plates are suspended into the broth with the help of wire loop.
4. After suspension this broth is kept on orbital shaker for multiplication of bacteria at 125 – 130 rpm for 5 days.
5. Kept NB on orbital shaker for microorganisms growth.
6. After a 5-day period, prepare a mixture by adding 200gm of jaggery to 20 liters of tap water in a container. Then, transfer 500 ml of NB (nutrient broth) containing microbial colonies into the mixture. Stir gently 2-3 times and incubate for an additional 5 days.
Result
Mass multiplication of BARC culture has been successfully accomplished.
