Date- 01/07/2022
Introduction :
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture.
Requirements :
For 1 lit Media preparation –
| Stock 1 | 25ml |
| Stock 2 | 1 ml |
| Stock 3 | 4ml |
| Stock 4 | 1 ml |
| Mynosyitol | 100 mg |
| Sucrose | 30 gm |
| Agar | 8 gm |
Procedure:
1. Take all stock solution in flask as per measurement .
2. Add 100mg mynosyitol .
3. Add 3o gm sucrose .
4. Add distill water Mix it well .
5. check Ph of Media if Ph is less than 5.8 then maintain it by using NaOH .
6. Add 8 gm Agar .
7. Now heat the media on flame till the agar gets completely dissolved .
8. Now pour the media into bottles and autoclave it .
Conclusion :
This media is use full for tissue culture plants .
